• 
  Fuminori Kizaki

  regizor

• 
  Takashi Okazaki

  scenarist

• 
  Samuel L. Jackson

  Afro Samurai / Ninja Ninja
• 
  Kelly Hu

  Okiku (voce)
• 
  Ron Perlman

  Justice
• 
  Jeff Bennett

  Foo / Hachiro (voce)
• 
  S. Scott Bullock

  Dharman (voce)
• 
  Grey Griffin

  Oyuki (voce)

• 
  Dwight Schultz

  Assassin #1  (1 episode, 2007)
• 
  Tara Strong

  Otsuru  (2 episodes, 2007)
• 
  Yuri Lowenthal

  Kuma  (5 episodes, 2007)
• 
  Crystal Scales

  Young Afro Samurai (2 episodes, 2007)
• 
  John Kassir

  Soshun (1 episode, 2007)
• 
  Dave Wittenberg

  Assassin  (2 episodes, 2007)
• 
  Phil LaMarr

  Brother 1  (5 episodes, 2007)

• 
  Jason Marsden

  Sasuke (2 episodes, 2007)
• 
  Liam O'Brien

  Kihachi  (1 episode, 2007)
• 
  Steve Blum

  Assassin (1 episode, 2007)
• 
  Terrence 'T.C.' Carson

  Sword Master  (3 episodes, 2007)
• 
  John DiMaggio

  Brother #2  (3 episodes, 2007)
• 
  James Arnold Taylor

  Yashichi (1 episode, 2007)
• 
  Fred Tatasciore

  Juzo  (2 episodes, 2007)
• 
  Greg Eagles

  Brother 6

• 
  Alex Gartner

  producător
• 
  Charles Roven

  producător