• 
  John Burgmeier

  Chikuma Koshiro
• 
  John Gremillion

  Muroga Hyoma
• 
  Shô Hayami

  Yakushiji Tenzen
• 
  Bill Jenkins

   Narrator (22 episodes, 2005)
• 
  Jason Liebrecht

  Kisaragi Saemon
• 
  Christopher Sabat

  Kasumi Gyobu
• 
  Paul Slavens

  Amayo Jingoro
• 
  Mark Stoddard

  Yakushiji Tenzen

• 
  Kenji Utsumi

  Mino Nenki
• 
  Misa Watanabe

  Akeginu
• 
  Kosuke Toriumi

  Gennosuke Kouga
• 
  Laura Bailey

  Oboro Iga
• 
  Risa Hayamizu

  Kagero
• 
  Miyuki Sawashiro

  Hotarubi
• 
  Colleen Clinkenbeard

  Hotarubi

• 
  Nana Mizuki

  Oboro Iga
• 
  Troy Baker

  Gennosuke Kouga
• 
  Katsuhiro Kitagawa

  Kasumi Gyobu
• 
  Wataru Hatano

  Chikuma Koshiro
• 
  Yoji Ueda

  Kisaragi Saemon
• 
  Stephanie Young

  Kagero
• 
  Yasushi Miyabayashi

   Narrator / ... (23 episodes, 2005)
• 
  Jim Johnson

  Mino Nenki
• 
  Jennifer Seman

  Akeginu
• 
  Ken Uo

  Amayo Jingoro