• 
  Fred M. Wilcox

  regizor

• 
  Allen Adler

  scenarist

• 
  Anne Francis

  Altaira Morbius
• 
  Leslie Nielsen

  Commander J. J. Adams
• 
  Walter Pidgeon

  Dr. Edward Morbius
• 
  Jack Kelly

  Jerry Farman
• 
  Richard Anderson

  Chief Quinn
• 
  Frankie Darro

  Robby the Robot

• 
  Les Tremayne

  Narator
• 
  James Best

  Crewman
• 
  Earl Holliman

  Cook
• 
  Harry Harvey Jr.

  Crewman Randall
• 
  Roger McGee

  Crewman Lindstrom
• 
  Warren Stevens

  Lt. 'Doc' Ostrow M.D.
• 
  Robert Dix

  Crewman Grey

• 
  James Drury

  Crewman Strong
• 
  Morgan Jones

  Crewman Nichols
• 
  Peter Miller

  Crewman Moran
• 
  Jimmy Thompson

  Crewman Youngerford
• 
  George Wallace

  Bosun