• 
  Hilary Haag

   Princess Sen (5 episodes, 2010)
• 
  Houko Kuwashima

   Chizuru Yukimura (12 episodes, 2010)
• 
  Shinichirô Miki

  Toshizo Hijikata
• 
  Shotaro Morikubo

  Souji Okita
• 
  Nobuo Tobita

   Keisuke Sannan (12 episodes, 2010)
• 
  Hiroyuki Yoshino

  Heisuke Toudou
• 
  Illich Guardiola

   Sanosuke Harada (12 episodes, 2010)
• 
  David Matranga

   Keisuke Sannan (12 episodes, 2010)

• 
  Kosuke Toriumi

  Hajime Saito
• 
  Kôji Yusa

   Sanosuke Harada (12 episodes, 2010)
• 
  Tôru Ôkawa

  Isami Kondou
• 
  Kenjirô Tsuda

   Chikage Kazama (7 episodes, 2010)
• 
  Greg Ayres

  Heisuke Toudou
• 
  Kurt Bauer

  Genzaburo Inoue
• 
  Clint Bickham

   Kashitaro Itou (6 episodes, 2010)

• 
  Blake Shepard

  Souji Okita
• 
  Brittney Karbowski

   Chizuru Yukimura (12 episodes, 2010)
• 
  Kalob Martinez

   Shinpachi Nagakura (12 episodes, 2010)
• 
  Andrew Love

  Toshizo Hijikata
• 
  Ryûsaku Chijiwa

   Kashitaro Itou (6 episodes, 2010)
• 
  Norio Kobayashi

  Genzaburo Inoue
• 
  Tomohiro Tsuboi

   Shinpachi Nagakura (12 episodes, 2010)
• 
  Steve Fenley

   Kyuju Amagiri (8 episodes, 2010)
• 
  Ryu Yamaguchi

   Kyuju Amagiri (8 episodes, 2010)
• 
  David Wald

  Isami Kondou
• 
  Ayano Ishikawa

   Princess Sen (5 episodes, 2010)
• 
  Corey Hartzog

   Chikage Kazama (7 episodes, 2010)
• 
  Hiroaki Yoshida

   Kyo Shiranui (6 episodes, 2010)
• 
  Connor Leach

   Susumu Yamazaki (7 episodes, 2010)
• 
  Houston Hayes

   Kyo Shiranui (6 episodes, 2010)
• 
  Takamasa Suzuki

   Susumu Yamazaki (7 episodes, 2010)
• 
  Leraldo Anzaldua

  12 episodes, 2010
• 
  Andrew Love

  12 episodes, 2010