• 
  Houko Kuwashima

  Chizuru Yukimura (voice)
• 
  Shinichirô Miki

  Toshizo Hijikata
• 
  Shotaro Morikubo

  Souji Okita
• 
  Illich Guardiola

  Sanosuke Harada (voice)
• 
  David Matranga

  Keisuke Sannan (voice)
• 
  Mark Laskowski

  Kodo Yukimura (voice) (as Mark X. Laskowski)
• 
  Tôru Ôkawa

  Isami Kondou
• 
  Greg Ayres

  Heisuke Toudou

• 
  Kurt Bauer

  Genzaburo Inoue
• 
  Brittney Karbowski

  Chizuru Yukimura (voice)
• 
  Kalob Martinez

  Shinpachi Nagakura (voice)
• 
  Andrew Love

  Toshizo Hijikata
• 
  Norio Kobayashi

  Genzaburo Inoue
• 
  Ryuugo Saito

  Kodo Yukimura (voice)
• 
  Leraldo Anzaldua

  Hajime Saito (voice)