• 
  Johnny Yong Bosch

  Jonathan Joestar
• 
  Yumiko Kobayashi

  Poco (voice)
• 
  Takehito Koyasu

  Dio Brando
• 
  Tamio Ôki

  Tonpeti (voice) (as Tamio Ooki)
• 
  Nobuo Tobita

  Straits (voice)
• 
  Tôru Ôkawa

  Narrator (voice)
• 
  Taketora

  Dire (voice)
• 
  Yoji Ueda

  Robert E. O. Speedwagon (voice)

• 
  Aya End

  Poco's Sister (voice)
• 
  Kazuyuki Okitsu

  Jonathan Joestar
• 
  Kenshirô Usuki

  Poco's Father (voice)
• 
  Paul St. Peter

  Dire / Wamu (voice)
• 
  Michael McConnohie

  Tonpetty (voice)
• 
  Kirk Thornton

  Dobbie (voice)
• 
  Karen Strassman

  Mother (voice)

• 
  Yuuichi Ishigami

  Doobie (voice)
• 
  Ray Chase

  Poco's Dad (voice)
• 
  Toru Sakura

  Man-Faced Dog (voice)
• 
  Johanna Luis

  Poco's Sister (voice)