• 
  Johnny Yong Bosch

  Jonathan Joestar
• 
  Ayako Kawasumi

  Erina Pendleton (voice)
• 
  Yumiko Kobayashi

  Poco (voice)
• 
  Takehito Koyasu

  Dio Brando
• 
  Hiroshi Naka

  Wang Chan (voice)
• 
  Tamio Ôki

  Tonpeti (voice) (as Tamio Ooki)
• 
  Nobuo Tobita

  Straits (voice)
• 
  Tôru Ôkawa

  Narrator (voice)

• 
  Yoji Ueda

  Robert E. O. Speedwagon (voice)
• 
  Aya End

  Poco's Sister (voice)
• 
  Kazuyuki Okitsu

  Jonathan Joestar
• 
  Christopher Hackney

  Styx (voice)
• 
  Doug Stone

  Wang Chen (voice)
• 
  Masayuki Katô

  Father Styx (voice)
• 
  Shinobu Matsumoto

  Priest (voice)

• 
  Hidenori Takahashi

  Man B (voice)