• 
  Emily Rose

   Gwen Stacy
• 
  Stan Lee

  Stan Lee
• 
  Jackson Buffington

  Peter Parker
• 
  Joy Pyper

  3 episodes, 2017
• 
  Shawn Buffington

  14 episodes, 2017-2018
• 
  Brendan Wreay

  Harry Osborn
• 
  Meggie Elise

  Gwen Stacy
• 
  Jacob M. Keene

  Kingpin

• 
  Thunder Muffin

  Hobgoblin
• 
  Chris Wilson

  Miles Morales
• 
  Chrissy Pixy

  Mary Jane Watson
• 
  Christopher Zuckerman

  Captain George Stacy
• 
  Eman Sdrawkcab

  Flash Thompson
• 
  Andrew Mondia

  Adrian Toomes
• 
  Carrick Inabnett

  Electro

• 
  Steve Stanley

  Roderick Kingsley
• 
  Mackenzie Bryant

  Gwen Stacy
• 
  Ryan Wichael

  Ned Leeds
• 
  Erik Smith

  Mac Gargan