• 
  John Ford

  regizor

• 
  Katharine Hepburn

  Mary Stuart
• 
  Fredric March

  Marchizul de Bothwell
• 
  William Stack

  Ruthven
• 
  Earle Foxe

  Earl of Kent
• 
  Billy Watson (II)

  Fisherman's son
• 
  Douglas Gerrard
• 
  Winter Hall

• 
  Wedgwood Nowell

  Queen Elizabeth's Majordomo
• 
  Shep Houghton

  Soldat
• 
  Jean Fenwick

  Mary Seton
• 
  Douglas Walton

  Darnley
• 
  Robert Barrat

  Morton
• 
  Barlowe Borland

  Judecător
• 
  Neil Fitzgerald

  Nobleman

• 
  Florence Eldridge

  Elizabeth Tudor
• 
  Doris Lloyd

  Fisherman's Wife
• 
  Walter Byron

  Walsingham
• 
  Lionel Belmore

  Fisherman
• 
  Frank Baker

  Douglas
• 
  Ivan F. Simpson

  Judecător
• 
  Alec Craig

  Donal
• 
  Robert Warwick

  Sir Francis Knollys
• 
  Halliwell Hobbes

  Bărbat
• 
  Alan Mowbray

  Throckmorton
• 
  Lawrence Grant

  Judecător
• 
  Donald Crisp

  Huntly
• 
  John Carradine

  Rizzio
• 
  Ian Keith

  Moray
• 
  David Clyde
• 
  Lionel Pape

  Burghley
• 
  David Torrence

  Lindsay
• 
  Ralph Forbes

  Randolph
• 
  Brandon Hurst

  Airan
• 
  Murray Kinnell

  Judecător
• 
  D'Arcy Corrigan

  Kirkcaldy
• 
  Jean De Briac

  Bărbat
• 
  Mary Gordon

  Nurse
• 
  Monte Blue

  Messenger
• 
  Frieda Inescort

  Mary Beaton
• 
  Harry Tenbrook

  One of Queen Mary's Guards
• 
  Gavin Muir

  Leicester
• 
  Moroni Olsen

  John Knox