• 
  Catherine Corcoran

  Lauren
• 
  Zac Amico

  Zack
• 
  Stan Lee

  narator
• 
  Mike Baez

  Donatello
• 
  Lloyd Kaufman

  Lee Harvey Herzkauf
• 
  Josh Potter

  Kevin the Duck Monster / Jock
• 
  Babette Bombshell

  Principal Westly
• 
  Clay von Carlowitz

  Eugene 'The Machine' McCormack

• 
  Stefan Dezil

  Slater
• 
  Vito Trigo

  Leonardo
• 
  Mark Quinnette

  Michaelangelo
• 
  Reiki Tsuno

  Rembrandt
• 
  Asta Paredes

  Chrissy Goldberg
• 
  Tara Miller

  Rachel Ruysch
• 
  Jim Sheppard

  Raphael

• 
  Lauren Sylvester

  Eugene's Tranny Friend