• 
  Matthew Lillard

  Shaggy Rogers
• 
  Jeffrey Combs

  The Question
• 
  Diedrich Bader

  Batman
• 
  Jeff Bennett

  Joker
• 
  Tara Strong

  Harley Quinn
• 
  Grey Griffin

  Daphne Blake
• 
  John DiMaggio

  Aquaman / Mr. Freeze (voice)
• 
  Nika Futterman

  Catwoman (voice)

• 
  Nicholas Guest

  Martian Manhunter
• 
  John Michael Higgins

  Riddler (voice)
• 
  Tom Kenny

  Penguin
• 
  Kevin Michael Richardson

  Detective Chimp (voice)
• 
  Fred Tatasciore

  Harvey Bullock (voice)
• 
  Frank Welker

  Fred Jones
• 
  Kate Micucci

  Velma Dinkley