• 
  Cecil B. DeMille

  regizor

• 
  Gary Cooper

  Wild Bill Hickok
• 
  Anthony Quinn

  Cheyenne Indian
• 
  Jean Arthur

  Calamity Jane
• 
  Frank McGlynn Sr.

  Abraham Lincoln
• 
  Ben Hendricks Jr.

  Trooper
• 
  Granville Bates

  Van Ellyn
• 
  Everett Brown

• 
  Hooper Atchley

  Căpitan
• 
  Bruce Warren

  Purser of the 'Lizzie Gill'
• 
  Lane Chandler

  Capt. Wood's trooper
• 
  Francis Ford (I)

  Anderson (old veteran)
• 
  Jane Keckley

  Deadwood woman yelling, 'You keep out of it!'
• 
  Purnell Pratt

  Capt. Wood
• 
  Irving Bacon

  Hysterical trooper

• 
  Porter Hall

  Jack McCall
• 
  Budd Fine
• 
  Francis McDonald

  River gambler
• 
  John Miljan

  Gen. George A. Custer
• 
  Charles Judels

  Tony (barber)
• 
  Wadsworth Harris

  William Dennison
• 
  Edgar Dearing

  Custer's messenger
• 
  Dennis O'Keefe

  Man #2
• 
  David Clyde

  Miner #2
• 
  Jim Mason

  Lattimer's second teamster
• 
  Stanley Andrews
• 
  Sherwood Bailey
• 
  Lona Andre

  Southern belle
• 
  Charles Bickford

  John Lattimer
• 
  Oscar Rudolph

  Younger man
• 
  George 'Gabby' Hayes

  Breezy
• 
  Fuzzy Knight

  Dave
• 
  Jonathan Hale

  Major
• 
  Edwin Maxwell

  Secretary of War Edwin Stanton
• 
  Richard Alexander

  Lattimer's third teamster
• 
  Monte Blue
• 
  Harry Stubbs

  John F. Usher
• 
  Louis Natheaux
• 
  Carl Miller

  Lattimer's wagond driver
• 
  Frank Albertson

  Young trooper
• 
  Hank Bell

  Capt. Wood's medic
• 
  Hank Worden

  Deadwood townsman
• 
  Paul Harvey

  Yellow Hand
• 
  Helen Burgess

  Louisa Cody
• 
  James Ellison

  Buffalo Bill Cody
• 
  Fred Kohler

  Jake - A Teamster (as Fred Kohler Sr. in End Credit)
• 
  Pat Moriarity

  Sergeant McGinnis (as Pat Moriarty)
• 
  Victor Varconi

  Painted Horse