• 
  Victor Cook

  regizor

• 
  Stan Lee

  scenarist

• 
  Josh Keaton

  Peter Parker
• 
  Lacey Chabert

  Gwen Stacey
• 
  Joshua LeBar

  Flash Thompson
• 
  Ben Diskin

  Eddie Brock
• 
  Deborah Strang

  Matusa May Parker
• 
  James Arnold Taylor

  Harry Osborn

• 
  Tricia Helfer

  Black Cat / Felicia Hardy
• 
  Stan Lee

  Stan
• 
  Jane Lynch

  Joan Jameson (1 episode, 2009)
• 
  Thom Adcox-Hernandez

  Phineas Mason  (4 episodes, 2009)
• 
  Courtney B. Vance

  Roderick Kingsley (1 episode, 2009)
• 
  James Remar

  The Cat Burglar  (1 episode, 2009)
• 
  Peter MacNicol

  Dr. Otto Octavius  (12 episodes, 2008-2009)

• 
  Xander Berkeley

  Quentin Beck  (4 episodes, 2008-2009)
• 
  Kath Soucie

  Martha Connors  (9 episodes, 2008-2009)
• 
  Cree Summer

  Glory Grant  (6 episodes, 2008-2009)
• 
  B.J. Ward

  Mayor Waters (1 episode, 2008)
• 
  Kelly Hu

  Sha Shan Nguyen  (5 episodes, 2009)
• 
  Charles Duckworth

  Hobie Brown  (1 episode, 2009)
• 
  Danny Trejo

  Ox (1 episode, 2009)
• 
  Thomas F. Wilson

  Stan Carter (6 episodes, 2008-2009)
• 
  Clancy Brown

  George Stacy  (16 episodes, 2008-2009)
• 
  Dee Bradley Baker

  Dr. Curt Connors  (9 episodes, 2008-2009)
• 
  Kevin Michael Richardson

  L. Thompson Lincoln  (12 episodes, 2008-2009)
• 
  Miguel Ferrer

  Silvermane  (2 episodes, 2009)
• 
  Irene Bedard

  Jean DeWolff (4 episodes, 2008-2009)
• 
  Robert Englund

  Adrian Toomes  (5 episodes, 2008-2009)
• 
  Max Burkholder

  Billy Connors (1 episode, 2008)
• 
  Vanessa Marshall

  Mary Jane Watson  (17 episodes, 2008-2009)
• 
  Alanna Ubach

  Liz Allan  (18 episodes, 2008-2009)
• 
  Edward Asner

  Uncle Ben Parker (1 episode, 2008)
• 
  Nikki Cox

  Sable Manfredi  (2 episodes, 2009)
• 
  Alan Rachins

  Norman Osborn (15 episodes, 2008-2009)
• 
  Keith David

  Big Man (1 episode, 2008)
• 
  Phil LaMarr

  Randy Robertson  (14 episodes, 2008-2009)
• 
  Bill Fagerbakke

  Morris Bench (1 episode, 2009)
• 
  Dorian Harewood

  Dr. Bromwell (4 episodes, 2008-2009)
• 
  Crispin Freeman

  Electro  (5 episodes, 2008-2009)
• 
  Steve Blum

  Green Goblin  (15 episodes, 2008-2009)
• 
  Robert Costanzo

  Sullivan Edwards (1 episode, 2008)
• 
  John DiMaggio

  Hammerhead  (12 episodes, 2008-2009)
• 
  Brian George

  Miles Warren  (7 episodes, 2008-2009)
• 
  Daran Norris

  J. Jonah Jameson  (13 episodes, 2008-2009)
• 
  Grey Griffin

  Sally Avril  (19 episodes, 2008-2009)
• 
  Elisa Gabrielli

  Dr. Ashley Kafka (2 episodes, 2008-2009)
• 
  Andrew Kishino

  Kenny 'King' Kong