• 
  Lisa Ann Beley

  Override
• 
  Garry Chalk

  Optimus Prime
• 
  Bill Mondy

  Evac
• 
  Richard Newman

  Vector Prime
• 
  Michael Adamthwaite

  Lugnutz
• 
  Tabitha St. Germain

   Professor Lucy Suzuki (15 episodes, 2005-2006)
• 
  Sarah Edmondson

   Lori (52 episodes, 2005-2006)
• 
  Sam Vincent

   Coby (52 episodes, 2005-2006)

• 
  Kirby Morrow

  Hot Shot
• 
  Ryan Hirakida

   Bud / ... (52 episodes, 2005-2006)
• 
  Brian Dobson

  Red Alert
• 
  Mark Oliver

  Thundercracker
• 
  Michael Dobson

  Starscream
• 
  Brian Drummond

  Jetfire
• 
  David Kaye

  Megatron